Part:BBa_K365010:Design

Phytochrome B (aa 1-642) linked to a trimer of ClpX (aa 61-425)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 490
  Illegal BglII site found at 2697
  Illegal BglII site found at 3861
  Illegal BglII site found at 5025
  Illegal BamHI site found at 572
  Illegal XhoI site found at 523
  Illegal XhoI site found at 542
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 739
  

Design Notes

We used Freiburg fusion standard (RFC-25) for in frame assembly.

Source

See subparts.